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From soybean matrix, glyphosate was extracted with a mixture of water and methanol (4:1, v/v) from soybean samples followed by protein precipitation with equal volume of methanol. No preconcentration and further clean
up of the sample were required. Pre-column derivatization was carried out with excess amount of 9-
fluorenylmethyl chloroformate (FMOC-Cl) in the presence of borate buffer.