Government of Nepal

Ministry of Education, Science and Technology

National Forensic Science Laboratory

Development of simple and sensitive HPLC method for determination of glyphosate residues in soybean

From soybean matrix, glyphosate was extracted with a mixture of water and methanol (4:1, v/v) from soybean samples followed by protein precipitation with equal volume of methanol. No preconcentration and further clean
up of the sample were required. Pre-column derivatization was carried out with excess amount of 9-
fluorenylmethyl chloroformate (FMOC-Cl) in the presence of borate buffer.

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